FELASA guidelines for the refinement of methods for genotyping genetically-modified rodents (2013)

Publication

FELASA guidelines for the refinement of methods for genotyping genetically-modified rodents: a report of the Federation of European Laboratory Animal Science Associations Working Group. Bonaparte D, Cinelli P, Douni E, Hérault Y, Maas M, Pakarinen P, Poutanen M, Lafuente MS, Scavizzi F; Federation of European Laboratory Animal Science Associations Working Group. Lab Anim. 2013 Jul;47(3):134-45. DOI: 10.1177/0023677212473918

 

The increasing number of available mutated mouse strains and their combinations, together with the increasing complexity in the targeting approaches used, reinforces the need for guidelines that will provide information about the mouse strains and the robust and reliable methods used for their genotyping. This information, however, should be obtained with a method causing minimal discomfort to the experimental animals. The present document summarizes the currently available methods for obtaining genotype information.